CARBOHYDRATE CHARACTERIZATION

Synthesis and Characterization of Some N-Heterocyclic Carbohydrate Derivatives

The nucleophilic bimolecular substitution on 1,2:3,4-di-O-isopropylidene-α-Dgalactopyranose with NH2-heterocyclic derivatives allows us to obtain some new compounds with potential biological activities. The characterization of them as well as a discussion of their reactivities toward sulfur analogues are present. Introduction The synthesis of heterocyclic compounds containing a carbohydrate moi...

Characterization of Carbohydrate Surface Markers on Mouse Embryonic Stem Cells

Glycosylation of proteins and lipids on cell surface have been shown to be important in maintaining pluripotency and stem cell fate in embryonic stem cells. Lectins have been widely used to characterize carbohydrate modifications on cell surface of embryonic stem cells to determine pluripotency and stem cell fate. In the present study, a panel of 14 lectins and carbohydrate antibodies was used ...

Characterization of hepatic lactogen receptor. Subcellular distribution and characterization of N-linked carbohydrate chains.

The types of carbohydrate chains present in a rat liver lactogenic hormone-binding receptor species with an Mr of 82,000, and in its hormone-binding subunits with Mr values of 40,000 and 35,000, were characterized using carbohydrate-chain-cleaving enzymes and affinity cross-linking. The subcellular distribution of lactogenic hormone-binding species was studied in organelle-enriched fractions. T...

Carbohydrate biosensors.

2.1.1 General Procedure 5988 2.1.2 Heparin Detection 5992 2.1.3 Carbohydrate Structures 5994 2.2 Lectin-Based Biosensors 5995 2.3 Glycoprotein and Glycosylation Biosensors 5997 2.4 Pathogen and Cancer-Detection Assays 5999 2.4.1 Pathogen Identification 5999 2.4.2 LPS Biosensors 6002 2.4.3 Cancer Diagnostics 6003 2.5 Carbohydrate Nanobiosensors 6003 2.6 Miscellaneous Carbohydrate Bioassays 6004 ...

Carbohydrate Microarray